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BioEdit: A user-friendly biological sequence alignment editor and analysis program for windows 95/98/NT. First report of Maize chlorotic mottle virus on sweet corn in Taiwan. Journal of Molecular Biology, 215, 403–410.ĭeng, T. Characterising maize viruses associated with maize lethal necrosis symptoms in sub Saharan Africa. Plant Pathology, 62, 741–749.Īdams, I.P., Braidwood, L.A., Stomeo, F., Phiri, N., Uwumukiza, B., Feyissa, B., Mahuku, G., et al. Use of next-generation sequencing for the identification and characterization of Maize chlorotic mottle virus and Sugarcane mosaic virus causing maize lethal necrosis in Kenya. M., Wangai, A., Kimani, E., Phiri, N., Reeder, R., Harju, V., Glover, R., Hany, U., Souza-Richards, R., Deb Nath, P., Nixon, T., Fox, A., Barnes, A., Smith, J., Skelton, A., Thwaites, R., Mumford, R., & Boonham, N.
The tentative name Maize-associated pteridovirus (MaPV) has been proposed for the virus in this study.Īdams, I. RNA2 contains three open reading frames (ORFs), one of which encodes for a putative movement protein, while the remaining two encode for putative products with no known function. RNA1 encodes for a polyprotein product containing putative viral methyltransferase, helicase and polymerase domains. Bioinformatic analysis of assembled reads yielded two contigs (5.8 and 2.7 kbp) that shared sequence homology with RNA1 and RNA2 of the dsRNA Japanese holly fern mottle virus (JHFMoV) (genus: Pteridovirus) from a single sample collected in the Arusha region. In a survey for viruses associated with maize in Tanzania, 35 samples were sequenced using an RNA-tag-seq metaviromics approach. However, their contribution to the expression of disease symptoms still requires extensive research.
Metaviromics studies of plant viruses have allowed for the discovery of novel viral diversity, with a number of these viruses having recently been shown to co-occur with MCMV and Potyviruses. Synergistic infections of Maize chlorotic mottle virus (MCMV) and members of the Potyviridae family are known to elicit MLND. Maize lethal necrosis disease (MLND) is currently threatening maize production in a large area of East Africa.